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A male-sterile mutation in soybean (Glycine max) affecting chromosome arrangement in metaphase plate and cytokinesis Biocell
Pires Bione,Nilton Cesar; Suely Pagliarini,Maria; Alves de Almeida,Leones.
A spontaneous male-sterile, female-fertile mutation affecting bivalent arrangement at the metaphase plate and cytokinesis was detected in line BR98-197 of the soybean breeding program developed by Embrapa - National Soybean Research Centre. Untill diakinesis, meiosis was normal with chromosome pairing as bivalents. From this phase, in several meiocytes, bivalents were not able to organize a single metaphase plate and remained scattered in the cytoplasm in a few or several groups. In these meiocytes, chromosomes segregated in both divisions giving rise to several micronuclei. However, the main cause of male sterility was the absence of cytokinesis after telophase II. Instead of the typical tetrads of microspores, four nucleate coenocytic microspores were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Meiosis; Metaphase plate; Cytokinesis; Male sterility.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200004
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Abnormal meiotic behavior in three species of Crotalaria PAB
Ferreira,Kátia; Torres,Giovana Augusta; Carvalho,Isabelle Vilela de; Davide,Lisete Chamma.
The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fluorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confirming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalaria; Cytogenetics; Cytomixis; Meiosis; Pollen grain viability.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200012
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Abnormal spindle orientation during microsporogenesis in an interspecific Brachiaria (Gramineae) hybrid Genet. Mol. Biol.
Mendes-Bonato,Andréa Beatriz; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
This paper reports a case of abnormal spindle orientation during microsporogenesis in an interspecific hybrid of the tropical grass Brachiaria. In the affected plant, prophase I was normal. In metaphase I, bivalents were regularly co-oriented but distantly positioned and spread over the equatorial plate. In anaphase I, chromosomes failed to converge into focused poles due to parallel spindle fibers. As a consequence, in telophase I, an elongated nucleus or several micronuclei were observed in each pole. In the second division, the behavior was the same, leading to polyads with several micronuclei. A total of 40% of meiotic products were affected. The use of this hybrid in production systems needing good-quality seeds is discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria; Interspecific hybrid; Meiosis; Spindle abnormality; Tropical grass.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100023
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Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products Genet. Mol. Biol.
Schneider,Marielle Cristina; Carraro,Bianca Pierina; Cella,Doralice Maria; Matiello,Rodrigo Rodrigues; Artoni,Roberto Ferreira; Almeida,Mara Cristina.
In this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric...
Tipo: Info:eu-repo/semantics/article Palavras-chave: B chromosome; Constitutive heterochromatin; Karyotype; Meiosis; Ag-NORs.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400023
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Botanical aspects of Heteropterys umbellata (Malpighiaceae): a cytological and palynological approach Anais da ABC (AABC)
Sousa,Saulo M.; Reis,Aryane C.; Gomes,Shaiany S.L.; Bernardo,Karina B.; Salimena,Fátima R.G.; Viccini,Lyderson F..
The genus Heteropterys is one of the major genera in Malpighiaceae. However, few cytological and palynological studies were reported. The present work described for the first time the chromosome number, heterochromatin pattern, meiotic behavior, pollen viability and palynological aspects of Heteropterys ubellata, a very spread species. One large Brazilian population was evaluated using conventional techniques for meiotic studies and acetolyse to access the pollen morphology. The species showed 2n = 20 chromosomes, normal meiotic development and viable pollens. Great blocks of heterochromatin were observed around the centromers. DAPI staining was positive for centroeric heterochroatin, hile CMA3 ark as observed just at terinal regions of one pair of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome number; Heterochromatin pattern; Heteropterys umbellata; Malpighiaceae; Meiosis; Pollen viability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400009
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C-Banding patterns and meiotic behavior in Hypsiboas pulchellus and H. Cordobae (anura, hylidae) JBAG
Baraquet,M; Salas,N.E; Martino,A.L.
In this work, we observed and evaluated C-banding patterns and meiotic behaviour of Hypsiboas pulchellus and H. cordobae. Twenty metaphasic cells per individual were analyzed, which were obtained from intestinal and testis cells using conventional and C-banding techniques. The two species presented 2n= 24 chromosomes. Pairs 1 and 8 to 12 were metacentric whereas pairs 2 to 7 were submetacentric. The centromeric region of all chromosomes was positively C-banded but the C-banding pattern varied between species. At meiosis, cells with 12 bivalents were observed. Bivalents usually presented two terminal chiasmata, although the largest pair presented only one terminal chiasma. The cytogenetic data reconfirms the diploid somatic chromosome number of H....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotypes; C-banding; Meiosis; Hypsiboas pulchellus; Hypsiboas cordobae.
Ano: 2013 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000100005
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Chromosome complement and meiosis in three species of the Neotropical bug genus Antiteuchus (Heteroptera, Pentatomidae, Discocephalinae) Genet. Mol. Biol.
Lanzone,Cecilia; Souza,Maria José de.
Orcein staining of spermatocytes was used to study the meiotic behavior of holocentric chromosomes in three member of the genus Antiteuchus (commonly known as stink bugs). We describe and illustrate the karyotype of Antiteuchus mixtus, A. sepulcralis and A. macraspis which were cytogenetically characterized as having a diploid number of 2n = 14 and an XY sex chromosome system showing pre-reductional meiosis for autosomes and post-reductional meiosis for sex chromosomes. These species were also shown to have a long diffuse stage during meiotic prophase I and aberrant harlequin-type meiocytes. The chiasma frequency was also analyzed for two of the three species studied.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Holokinetic chromosomes; Sex chromosomes; Meiosis; Diffuse stage.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100011
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Chromosome number and microsporogenesis of two accessions of Brachiaria dura Stapf (Poaceae) Biota Neotropica
Risso-Pascotto,Claudiceia; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
The two accessions of B. dura analyzed (DU01 and DU02) are hexaploid (2n = 6x = 54), derived from x = 9. Meiotic abnormalities, such as precocious chromosome migration to the poles, laggards and micronuclei, were recorded in low frequency in both accessions. The few multivalent chromosome association at diakinesis and meiotic stability suggested that hexaploidy probably resulted from chromosome doubling. In DU02, chromosome transfer (cytomixis) among meiocytes, involving part or the entire genome was observed. The implication of these findings for the Brachiaria breeding is discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria dura; Meiosis; Chromosome number; Cytomixis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032009000200024
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Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE) Ciência Rural
Kiihl,Paulo Roberto Peres; Pereira,Andréia Rodrigues Alonso; Godoy,Sara Mataroli de; Stenzel,Neusa Maria Colauto; Risso-Pascotto,Claudicéia.
Almost 90% of species of the genus Passiflora are native to the American continent, with high commercial value due to the fact that some species are used for human food while others have ornamental and medical qualities. Passiflora serrato-digitata is one of the species that integrates the Paraná Agronomic Institute germoplasm bank at its experimental base in Londrina, PR, Brazil. Collected flower buds were fixed in ethanol/acetic acid (3:1 v/v) for 24h, transferred to 70% alcohol and stored under refrigeration. Slides were prepared by the squashing technique and stained with 1.0% propionic carmine; they were analyzed under an optic microscope. Irregularities in the chromosome segregation process of P. serrato-digitata have been verified by meiotic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsporogenesis; Meiosis; 2n gametes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000600016
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Chromosomes of Theridiidae spiders (Entelegynae): interspecific karyotype diversity in Argyrodes and diploid number intraspecific variability in Nesticodes rufipes Genet. Mol. Biol.
Stavale,Leila Miguel; Schneider,Marielle Cristina; Araujo,Douglas; Brescovit,Antonio Domingos; Cella,Doralice Maria.
Theridiidae is a derived family within the Araneoidea clade. In contrast to closely related groups, the 2n(male) = 20+X1X2 with acro/telocentric chromosomes is the most widespread karyotype among the theridiid spiders. In this work, the cytogenetic analysis of Argyrodes elevatus revealed original chromosome features different from those previously registered for Theridiidae, including the presence of 2n(male) = 20+X with meta/submetacentric chromosomes. Most individuals of Nesticodes rufipes showed family conserved karyotype characteristics. However, one individual had a 2n(male) = 24 due to the presence of an extra chromosome pair, which exhibited regular behavior and reductional segregation during meiosis. After silver staining, mitotic cells exhibited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome morphology; Cytogenetic; Meiosis; Nucleolar organizer region; Sex chromosome system.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400012
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Combined association of Presenilin-1 and Apolipoprotein E polymorphisms with maternal meiosis II error in Down syndrome births Genet. Mol. Biol.
Bhaumik,Pranami; Ghosh,Priyanka; Ghosh,Sujay; Feingold,Eleanor; Ozbek,Umut; Sarkar,Biswanath; Dey,Subrata Kumar.
Abstract Alzheimer's disease and Down syndrome often exhibit close association and predictively share common genetic risk-factors. Presenilin-1 (PSEN-1) and Apolipoprotein E (APOE) genes are associated with early and late onset of Alzheimer's disease, respectively. Presenilin −1 is involved in faithful chromosomal segregation. A higher frequency of the APOE ε4 allele has been reported among young mothers giving birth to Down syndrome children. In this study, 170 Down syndrome patients, grouped according to maternal meiotic stage of nondisjunction and maternal age at conception, and their parents were genotyped for PSEN-1 intron-8 and APOE polymorphisms. The control group consisted of 186 mothers of karyotypically normal children. The frequencies of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome; Genetic polymorphism; Karyotype; Meiosis; Microsatellite markers.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400577
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Comportamento citológico atípico durante a microsporogênese em Brachiaria ruziziensis e B. decumbens. Infoteca-e
RISSO-PASCOTTO, C.; MENDES-BONATO, A. B.; PAGLIARINI, M. S.; VALLE, C. B. do.
Em gramíneas forrageiras do gênero Brachiaria, populações poliplóides naturais são abundantes e geralmente apomíticas, enquanto as diploídes, que se reproduzem sexualmente, são raras. Considerando-se a estreita correlação existente entre poloploidia e apomixia neste gênero, e a necessidade de identificação de genótipos sexuais compatíveis para o sucesso de hibridação, conhecimentos de características citológicas básicas como modo de reprodução, comportamento meiótico e nível de ploidia tornam-se essenciais para direcionar os programas de melhoramento. Durante análises desenvolvidas na Universidade Estadual de Maringá, com acessos de várias espécies de uma coleção de germoplasma, mantida na Embrapa Gado de Corte, três acessos, sendo um B....
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Braquiária; Feed grasses.; Brachiaria Decumbens; Brachiaria Ruziziensis; Citogenética Vegetal; Gramínea Forrageira; Meiose; Poliploidia.; Cytogenetics; Polyploidy.; Meiosis.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/324062
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Comportamento mitótico e meiótico de cromossomos holocêntricos &gama; irradiados de Rhynchospora pubera (Cyperaceae) Biological Sciences
Vanzela, André Luís Laforga; UEL; Colaço, Waldeciro; UFPE.
Para avaliar a ocorrência de cinetócoros difusos em Rhynchospora (Cyperaceae), cromossomos de R. pubera foram fragmentados ou fundidos por irradiação &gama;. Este material foi investigado nas gerações celulares subseqüentes, em ambas, mitose e meiose. Alterações no número e no tamanho dos cromossomos foram detectadas, mas o comportamento dos cromossomos irradiados foi normal e nenhum micronúcleo foi observado. Todos os dados apontam para a presença de cinetócoros difusos em R. pubera.
Palavras-chave: 2.00.00.00-6 Ciências Biológicas Cyperaceae; Holocentric chromosomes; Meiosis; Mitosis; Rhynchospora 2.00.00.00-6 Ciências Biológicas.
Ano: 2002 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2364
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Comportamiento meiótico de diferentes especies de lulo, Solanum sp Acta Agron. (Palmira)
Pareja Ordóñez,Nancy Maricela; Santacruz Ordóñez,Nazira Katherine; Ordóñez Jurado,Héctor Ramiro; Lagos Burbano,Tulio César.
Se realizó un análisis del comportamiento meiótico de las especies de lulo S. hirtum, S. quitoense y S. sessiliflorum, siguiendo la metodología convencional para los estudios de microsporogénesis. Se tomaron botones florales en diferentes estados de desarrollo, fijándolos por 24 horas en una solución de tres partes de etanol por una parte de ácido acético, saturada con trazas de cristales de cloruro férrico. Para la preparación de las placas se siguió la técnica de aplastamiento, se liberaron las células madres del grano de polen y finalmente se hicieron las observaciones bajo microscopía de luz. El análisis mostró que la meiosis se presenta en longitudes de antera que van desde los 2,79 mm hasta los 4,45 mm. La normalidad meiótica fue del 100%, tanto para...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meiosis; Variabilidad; Microsporogénesis; Índice meiótico; Normalidad meiótica.
Ano: 2010 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122010000400002
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Cytogenetic analysis of Astylus antis (Perty, 1830) (Coleoptera, Melyridae): Karyotype, heterochromatin and location of ribosomal genes Genet. Mol. Biol.
Mendes-Neto,Ernani de Oliveira; Vicari,Marcelo Ricardo; Campaner,Carlos; Nogaroto,Viviane; Artoni,Roberto Ferreira; Almeida,Mara Cristina.
Cytogenetic analysis of Astylus antis using mitotic and meiotic cells was performed to characterize the haploid and diploid numbers, sex determination system, chromosome morphology, constitutive heterochromatin distribution pattern and chromosomes carrying nucleolus organizer regions (NORs). Analysis of spermatogonial metaphase cells revealed the diploid number 2n = 18, with mostly metacentric chromosomes. Metaphase I cells exhibited 2n = 8II+Xyp and a parachute configuration of the sex chromosomes. Spermatogonial metaphase cells submitted to C-banding showed the presence of small dots of constitutive heterochromatin in the centromeric regions of nearly all the autosomes and on the short arm of the X chromosome (Xp), as well as an additional band on one of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sex chromosomes; Meiosis; Metaphase; FISH; 18S rDNA.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200007
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Cytogenetic studies of three Lycosidae species from Argentina (Arachnida, Araneae) Genet. Mol. Biol.
Chemisquy,María A.; Gil,Sergio G. Rodríguez; Scioscia,Cristina L.; Mola,Liliana M..
Cytogenetic studies of the family Lycosidae (Arachnida: Araneae) are scarce. Less than 4% of the described species have been analyzed and the male haploid chromosome numbers ranged from 8+X 1 X 2 to 13+X 1 X 2 . Species formerly classified as Lycosa were the most studied ones. Our aim in this work was to perform a comparative analysis of the meiosis in " Lycosa " erythrognatha Lucas, " Lycosa " pampeana Holmberg and Schizocosa malitiosa (Tullgren). We also compared male and female karyotypes and characterized the heterochromatin of " L. " erythrognatha . The males of the three species had 2n = 22, n = 10+X 1 X 2 , all the chromosomes were telocentric and there was generally a single chiasma per bivalent. In " Lycosa " pampeana , which is...
Palavras-chave: Meiosis; C-banding; Fluorochrome staining; Karyotype; Spiders; "Lycosa" and Schizocosa.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500010
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Cytogenetic study of the genus Cousinia (Asteraceae, section Serratuloideae) in Iran Genet. Mol. Biol.
Sheidai,Masoud; Mehdigholi,Kazem; Ghahreman,Ahmad; Attar,Farideh.
Meiotic studies of ploidy level, chromosome paring and chiasma frequency were performed on 11 Cousinia (Asteraceae) species of the section Serratuloideae. The diploid number of the species studied was 2n = 2x = 24 and 26 so these species possess two different basic numbers (x = 12 or 13), a phenomenon common to other sections of the genus. The chromosome numbers of 9 species are reported here for the first time. When the 2n = 24 and 2n = 26 species were subjected to cluster analysis based on relative meiotic characters two different clusters were formed indicating their distinctness. Our data support the results obtained from morphometry, anatomy, pollen morphology and molecular studies of the genus Cousinia.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cousinia; Chiasma frequency; Cluster analysis; Meiosis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100022
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Cytological analysis of hybrids among triticales and trigopiros Genet. Mol. Biol.
Fradkin,Maia; Greizerstein,Eduardo; Paccapelo,Héctor; Ferreira,Víctor; Grassi,Ezequiel; Poggio,Lidia; Ferrari,María Rosa.
We studied three different tricepiros: (Don Santiago x Don Noé), (Cumé x Horovitz) and (Cumé x Don Noé). The tricepiro (Don Santiago x Don Noé) was obtained by crossing the triticale Don Santiago INTA (AABBRR, 2n = 6x = 42) with the trigopiro Don Noé INTA (AABBDDJJ, 2n = 8x = 56). The number of chromosomes for the F1 was 2n = 49, the most frequent meiotic configuration being 14 bivalents and 21 univalents. The univalents were situated in the periphery of the equatorial plane, whereas the bivalents were located in the central zone. The chromatids in some of the univalents split when bivalents underwent reductional division in anaphase I. There were few laggard chromosomes or chromatids at this phase. The number of chromosomes (2n = 48-58) was high and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hybrids; Meiosis; Tricepiro; Univalents; Reductional division.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400022
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Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes Arq. Bras. Med. Vet. Zootec.
Adona,P.R.; Leal,C.L.V..
The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011
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Estudio de los cromosomas mitóticos y meióticos del sotol (Dasylirion cedrosanum Trel.) Phyton
Hernández-Quintero,JD; Reyes-Valdés,MH; Mendoza-Rodríguez,DV; Gómez-Martínez,M; Rodríguez-Herrera,R.
El género Dasylirion es un grupo de plantas típicas del Desierto Chihuahuense, perennes, de un comportamiento sexual dioico y denominadas comúnmente sotoles. Es un género poco estudiado desde el punto de vista biológico y se desconocen las bases de su dinámica reproductiva. En este trabajo se estudió el número cromosómico y el comportamiento meiótico de Dasylirion cedrosanum en el municipio de Saltillo, Coahuila, una localidad del noreste de México. Para la preparación de cromosomas mitóticos se utilizó una técnica basada en un tratamiento enzimático con pectoliasa y celulasa, así como tinción con acetocarmín. Para el estudio meiótico se recolectaron botones florales masculinos, se fijaron y se tiñeron con el mismo colorante. Como resultado, se reportan...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dasylirion cedrosanum; Dioecia; Cromosomas; Mitosis; Meiosis.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000100015
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